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KMID : 0624620080410100722
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BMB Reports
2008 Volume.41 No. 10 p.722 ~ p.727
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Proteomic analysis of androgen-independent growth in low and high passage human LNCaP prostatic adenocarcinoma cells
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Youm Yun-Hee
Kim Se-Yoon
Bahk Young-Yil
Yoo Tag-Keun
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Abstract
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The present study compared the proteomic characteristics of a low passage number (L-33) and high passage number (H-81) LNCaP cell clone. Marked differences in protein expression were noted in the response of L-33 and H-81 cells to androgens. To investigate if regulation of these proteins was androgen-dependent, expression of the androgen receptor was silenced via small interfering RNA. Consistent with the proteomic data, abrogation of androgen receptor production in H-81 cells resulted in the reversed expression level into L-33 cells compared with non-treated H-81 LNCaP cells. The results clarify the progression into an androgenindependent phenotype.
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KEYWORD
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Androgen receptor, Gene silencing, LNCaP cell model, Prostate cancer, Proteomics
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